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mirVana™ miRNA Isolation Kit P/N AM1560 The mirVana miRNA Isolation Kit (patent pending) is designed especially for the isolation of small RNAs, such as microRNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells. The kit uses a fast and efficient glass fiber filter (GFF) based procedure to isolate I'm using mirVana miRNA isolation kit. I had total RNA from 9 different lymphoid cell lines and I used the protocol FII to enrich for small RNAs - after total RNA extraction with trizol. After that, I analysed the purity of RNA in the Agilent 2100 Bioanalyzer, and for all cell lines, except one, I got a rRNA contamination between 6-15%, witch ... Total RNA was extracted using a mirVana miRNA isolation kit (Ambion, Austin, TX, USA), and quantified using an RNA BR assay and spectrophotometric analysis (Invitrogen, Carlsbad, CA, USA). The relative expression levels of miRNA-21 were measured using a two-step TaqMan assay according to the manufacturer's instructions (Applied Biosystems ... 1. KIT CONTENTS 2. DESCRIPTION The ISOLATE II miRNA kit allows convenient processing of multiple samples in 25 minutes without the use of laborious methods such as CsCl ultracentrifugation or handling of toxic chemicals such as phenol/chloroform. The ISOLATE II miRNA Kit is specially developed for the rapid phenol free isolation of highly Approximately 1 mg of each liver tissue sample stored in liquid nitrogen was ground with a homogenizer while still frozen, and total RNA containing miRNA was isolated according to the protocol of the mirVana miRNA Isolation kit (Ambion, Austin, TX) and stored at −80°C until analysis. miRNA expression levels were quantitated using the TaqMan ...

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Aging-associated changes in microRNA expression profile of internal anal sphincter smooth muscle: Role of microRNA-133a Jagmohan Singh,1 Ettickan Boopathi,2 Sankar Addya,3 Benjamin Phillips,4 Isidore Rigoutsos,5 RNeasy kits (discussed below in more detail) is the most commonly cited RNA extraction method. Besides RNeasy kits, QIAGEN RNA extraction products cited in the survey include QIAzol (optimized for fatty tissues) , RNase-free DNase and AllPrep DNA/RNA Micro Kit [56, 57], miRNeasy kit [10, 35, 58], Oligotex kit [59, 60], mRNA isolation ... Mirneasy Mini Handbook The RNeasy Plus Mini Kit purifies up to 100 µg total RNA (_200 nt) from a single extraction with efficient gDNA removal. The RNeasy Plus Micro Kit is specially. The PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18. Dec 23, 2013 · Qiagen, Hilden, Germany). Two different approximations were applied in mirVana kit: one following the exact manufacturer’s instructions and the other, a modified version of the centrifugation step to 30 min at 4°C after the phenol/chloroform step in order to increase the final RNA yields. The miRNA Isolation Kit provides a quick and easy spin column system for purifying and enriching micro RNAs (miRNAs) and other small cellular RNAs from a wide variety of tissue and cells. Since miRNAs are vital for regulating gene expression, this kit is optimized for isolation of small RNA molecules while removing larger RNAs and minimizing ... Small RNA isolation Small RNAs were isolated from 15 different mouse tissues [brain, heart, liver, spleen, lung, kidney, stomach, small intestine, colon, ovary, uterus, and developing testes at post natal day 7 (P7), P14, P21, and adult (8–10 weeks of age)] using a mirVanaTMP miRNA isolation kit (Ambion) according to the

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Total RNA was isolated using the mirVana miRNA (Ambion/Thermo Fisher) isolation kit, according to the manufacturer's recommendations. .. To evaluate miR‐155 expression in the samples, miR‐155 quantitative polymerase chain reaction (qPCR) was performed using miRCURY LNA Universal RT miRNA polymerase chain reaction (PCR) primer sets from Exiqon. Mar 05, 2019 · Total miRNA was extracted from exosomes using mirVana miRNA Isolation kit (Ambion; Thermo Fisher Scientific, Inc.). Total RNA was treated with TURBO DNase (Ambion; Thermo Fisher Scientific, Inc.) and cDNA was produced using the RT kit and was conducted according to the manufacturer's protocol. (Takara Biotechnology Co., Ltd., Dalian, China). In brief, the assay includes 2 steps: stem-loop RT and real-time PCR. Micro RNAs were isolated using the mirVana miRNA kit (Ambion). Stem-loop RT primers bind to at the 3′ portion of miRNA molecules and are transcribed with reverse transcriptase using a kit (Cat # 4366596, ABI). The MagMAX ™ mirVana Total RNA Isolation Kit is designed for isolation of total RNA, including microRNA, from a wide variety of sample matrices. The kit uses MagMAX™ magnetic-bead technology, ensuring reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan® miRNA Detection Assays. Conoco phillips 76 msds sheets. sheets Box 4428 Houston, TX 77210. After years of experience Power Lube Industrial phillips has come to carry over 200 of the most commonly- used lubricants today. Davis conoco Oil supplies commercial lubricants for use in crankcases transmissions gear lubricants as sheets well conoco as lubricating phillips greases.

2. miRNA Wash Solution contains guanidinium thiocyanate which is a potentially hazardous substance and should also be used in the fume hood. VIII. Materials and Equipment Name Supplier Number: # Model or Catalogue # AllPrep Kit Mini Qiagen 80204 mirVana miRNA Isolation Kit/ mirVana miRNA Isolation Kit PCI omitted Applied We resuspend exosomes from one tube of blood (~10 ml human blood and ~2 ml mouse blood) in 300 μl RNA lysis buffer from the mirVana miRNA isolation kit. To purify exosomes from cell culture media, the media is spun at 500 x g for 10 min to remove cells and also filtered through a 0.22 μm filter after centrifugation at 12,000 x g.

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Aug 24, 2010 · Abstract. The effect of RNA silencing in plants can be amplified if the production of secondary small interfering RNAs (siRNAs) is triggered by the interaction of microRNAs (miRNAs) or siRNAs with a long target RNA. miRNA and siRNA interactions are not all equivalent, however; most of them do not trigger secondary siRNA production. Briefly, mRNA and miRNA fractions were isolated from the purified SMCs from the circular smooth muscle (CSM) layer of the IAS as previously described , by using miRVana miRNA isolation kit following the manufacturer's protocol . These studies were performed at the Functional Genome Centre of Thomas Jefferson University.